Objective Focusing

  1. Focus the beam through the objective (pink) using the f200 lens: slide the lens along the rails to adjust the distance between the lens and objective until the beam path passes through the infinity corrected objective.

  1. Place the xyz-stage (orange) on top of the objective. If necessary, use support cubes to reinforce the structure.

  1. Direct the beam through the appropriate set of filters and dichroic mirror, mounted in the filter cube (yellow), and up through the objective.